On-Chip DNA Methylation Analysis Using Osmium Complexation

نویسندگان

  • Kaori Sugizaki
  • Tadashi Umemoto
  • Akimitsu Okamoto
چکیده

The development of a reaction for detecting the presence/absence of one methyl group in a long DNA strand is a chemically and biologically challenging research subject. A newly designed chemical assay on a chip for the typing of DNA methylation has been developed. A methylation-detection probe fixed at the bottom of microwells was crosslinked with methylated DNA mediated by osmium complexation and contributes to selective amplification of methylated DNA.

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عنوان ژورنال:

دوره 2011  شماره 

صفحات  -

تاریخ انتشار 2011